CSC students gain science research experience

CHADRON -- How do over the counter medications affect behavior in a Madagascan Hissing Cockroach? How effective are masks used by wildland firefighters? What happens when a medicine that raises blood pressure is administered to living heart tissue?
Original findings on these and a variety of other subjects formed the basis of presentations by 24 Chadron State College physical science students at the 135th annual meeting of the Nebraska Academy of Sciences conference in April.
The CSC contingent presenting at this year’s NAS conference was the largest in several years and follows the recent growth in students majoring in physical science, according to Dr. Mary Keithly, associate professor of chemistry. The degree requires a research component and a conference presentation, she said.
The projects begin in a student’s junior year when they work with a mentor to select a topic that allows collection of quantitative data and contributes new information to a field of study. In their senior year the students collect and analyze the data and write a thesis that they present at the conference.
The research that Crimsen Blankenship, of Rangely, Colorado, conducted on the effect of hallucinogenic pharmaceuticals on animal behavior began last fall when CSC purchased the cockroaches (Gromphadorhina Portentosa) as a possible alternative to fruit flies for studying neurological affects, said Keithly.
The two to three inch long insects are good for research because they are easy to observe and docile with people but loud and territorial, according to Dr. Jeffrey Kiiskila, assistant biology professor.
As it turns out the roaches may not be useful subjects for the type of project that Blankenship conducted. Although they became more aggressive on Benadryl and more passive on Robitussin, the roaches showed little interest in running a maze to test the drugs’ effect on memory, Kiiskila said.
“It may point to the inability to use them as a model if you cannot get them motivated by food to run any trials,” said Keithly.
But the project still had educational value, according to Kiiskila.
“The quality of the research was good for a starter experiment. Philosophically (Blankenship) learned that Benadryl did not align with our expectations whereas Robitussin did,” he said. “I think the biggest take away is learning about experimental design…and what to do when things go wrong.”
For his investigation into the effects of drugs like adrenaline on heart tissue Elijah Huntington, of Rapid City, S.D, used thinly sliced segments of a freshly harvested rat heart which he placed in an oxygenated solution with a device that delivered an electrical current to keep the tissue alive by mimicking a beating heart.
Huntington created the device, called a Bioelectrophysiological Cardiac Model Apparatus (BECMA) himself, based on a design he found, said Keithly. His BECMA cost half the price of the original design, she added.
Huntington’s experiments showed predicted increases in heart function with the drug treatments, and that tissues treated repeatedly showed faster declines, according to Keithly. Further studies are needed to confirm these findings and complete a full statistical analysis, she said.
Among other research topics were a study on firefighter face mask effectiveness by Alexis Kramer, of St. Paul, and an analysis by Morgan Ekwall of Yoder, Wyoming on how a person’s head movement is affected by the way they walk.
Keithly said research projects combine students’ knowledge and lab skills gained from CSC coursework to real world problems and presenting at the conference provides them with important communication skills.
Students also realize that conducting research and presenting at the conference has lasting value, said Keithly.
“Developing and completing their own research projects will make them more competitive in their graduate or professional programs,” she said.
Students and their presentation titles
Brady Abbott of Chadron: pXRF of the ADE collection
Crimsen Blankenship of Rangely, Colo.: Physiological and behavioral effects of over-the-counter hallucinogens on Gromphadorhina portentosa
Paige Boitz of Pueblo West, Colo.: Bioaccessibility of macronutrients in organic vs conventional food
Chance Cooper of Bridgeport, Neb.: The pyrolysis paradigm: transforming plastic waste into valuable resources
Morgan Ekwall of Torrington, Wyo.: The effects pronation and supination of the feet have on lateral head tilt
Maddison Frohling of Rapid City, S.D.: The effects of mental and physical activity on speech language delays
Aiyana Fujiyama of Wahiawa, Hawaii: Uranium absorption in produce
Kaylea Geiser of Syracuse, Neb.: Investigating toothbrush hygiene practices: storage and decontamination methods for disease prevention in daily oral care
Natalie Hamaker of Overton, Neb.: Sequencing the genome of a Borrelia burgdorferi strain found in Thurston County, Nebraska
Xavier Harrell of Highlands Ranch, Colo.: The effects of calcaneal pronation and supination on the posture of the shoulders
Colton Holoubeck of Kearney, Neb.: Examination of insulin degradation
Elijah Huntington of Rapid City, S.D.: Cardiotoxic effects of vasopressors on in vitro myocardial tissue
Ashley Jansen of Box Elder, S.D.: The use of nitrogen-based fertilizers on tomato plants
Christopher Jennings of O'Neill, Neb.: Microbial harmonies: investigating the influence of music genres on E. coli growth
David Johnson of Chadron: Nanoparticle-based biosensing assay for multiplexed detection of circulating transcripts for early detection of PDAC
Alexis Kramer of Saint Paul, Neb.: Zerumbone as a GLI-1 inhibitor in triple-negative breast cancer cells
Kendall Petty of Loveland, Colo.: Analyzing the effectivity of wildland firefighter masks
Aubree Quast of Clarks, Neb: Black cumin as an antimicrobial agent
Bailey Scherbarth of Hay Springs, Neb.: Analysis of IGF-1 and anti-inflammation on in vitro muscle healing
Conner Schwend of Saint Xavier, Mont.: Turmeric threads – dying to change color
Joselyn Schiaffo of Hay Springs, Neb.: Studying different infant formulas and breastmilk and their effects of fluorosis and/or decay on teeth
Autumn Skow of Midland, S.D.: Analysis of the hoof structure with jojoba oil and aloe vera
Thomas Walsh of Kearney, Neb.: Electroculture of tomato plants
Hunter Wiebelhaus of Springview, Neb.: Effects of apple cider vinegar on BMI
Category: Campus News, Physical and Life Sciences